Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
The following medicines may interact with prednisolone:
Do not stop giving your pet these drugs abruptly, as this can have life-threatening consequences. Ask your vet about weaning your dog off of them slowly. You should let your veterinarian know if your dog is on any other medications, as these can react badly with prednisone or prednisolone. A vet should be consulted for dogs that are pregnant, and puppies that are too young should not get these drugs as they can inhibit growth and cause other problems. Diabetic dogs should also not take them. Overdose can lead to itching, seizures, loss of hearing, weakness, anxiety, depression, high blood pressure, or heart problems. Follow your veterinarian’s dosage guidelines and contact your vet if you see signs of any serious symptoms.